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Image Search Results
Journal: bioRxiv
Article Title: Raman2RNA: Live-cell label-free prediction of single-cell RNA expression profiles by Raman microscopy
doi: 10.1101/2021.11.30.470655
Figure Lengend Snippet: A general-purpose microscope control software Micro-manager and a custom MATLAB script were combined to enable automated multi-modal measurements. Under Micro-manager, a Raman channel was registered as a ‘dummy’ channel along with brightfield and fluorescence channels. Micro-manager was responsible for changing the field of view (FOV) and imaging modality. During the Raman sequence, Micro-manager communicated with a digital acquisition (DAQ) board, through which a transistor-to-transistor logic (TTL) signal was generated to initiate the scanning sequence. Upon detection of the TTL signal, the MATLAB script controlled the Raman detector, laser shutter, and updated the galvo mirror angles through the DAQ board.
Article Snippet: Lastly, as smFISH consisted of 3 rounds of hybridization and imaging, the following steps were performed to register images across different rounds with a custom MATLAB script: Maximum intensity projection of nuclei stain and RNA images Automatic registration of round 1 images to rounds 2 and 3 based on nuclei stain images and
Techniques: Microscopy, Control, Software, Fluorescence, Imaging, Sequencing, Generated